Respiration in Single-Celled Organisms (AQA A-Level Biology): Flashcards

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Respiration in Single-Celled Organisms
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Type of organism used in respiration practical

Single-celled organism (yeast)

Role of methylene blue in respiration practical

Electron acceptor (redox indicator)

Methylene blue colour change during respiration

Blue to colourless

Independent variable (yeast respiration)

Temperature

Dependent variable (yeast respiration)

Time for methylene blue to become colourless

Formula: rate of respiration

1mean time\frac{1}{\text{mean time}}

Why buffer the yeast-glucose solution?

Maintain constant pH (prevents confounding enzyme effects)

Equilibration time in water bath

10 minutes

Yeast respiration at optimum temperature

Highest rate of respiration

Effect of very high temperature on enzymes

Denaturation (reduces/stops respiration)

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