Cell Fractionation & Ultracentrifugation (AQA A-Level Biology): Quizzes
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Practise the questions
10 questions from this quiz
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Practise the questions
10 questions from this quiz
What is cell fractionation?
What is cell fractionation?
A technique to separate cell organelles
Why must the solution be cold during cell fractionation?
Why must the solution be cold during cell fractionation?
To reduce enzyme activity
Why must the solution have the same water potential as the tissue?
Why must the solution have the same water potential as the tissue?
To prevent organelles bursting or shrinking
Why must the solution be buffered?
Why must the solution be buffered?
To maintain a stable pH
What is the mixture called that results from homogenation?
What is the mixture called that results from homogenation?
Homogenate
During ultracentrifugation, which organelles sink faster?
During ultracentrifugation, which organelles sink faster?
Heavier organelles
In what order do organelles separate during ultracentrifugation?
In what order do organelles separate during ultracentrifugation?
Nuclei, mitochondria, lysosomes
At what speed are mitochondria separated during centrifugation?
At what speed are mitochondria separated during centrifugation?
3,500 rpm
What is the supernatant?
What is the supernatant?
The liquid portion at the top of tube
What forms at the bottom of the tube after centrifugation?
What forms at the bottom of the tube after centrifugation?
A sediment or pellet