Manipulating Genomes (OCR A-Level Biology A): Flashcards

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Genetic Engineering
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Genetic engineering

Genome modification using techniques not possible via breeding

Why universal genetic code matters in genetic engineering

Gene from one species produces same protein in any other species

Restriction enzyme function

Cut DNA at specific sequences called restriction sites

Sticky ends

Unpaired sequences enabling complementary base pairing

Ligase enzyme function

Forms phosphodiester bonds between DNA fragments

Reverse transcriptase function

Synthesises DNA using RNA template

Major advantage of cDNA

Contains only exons, no introns - functional in bacteria

Recombinant DNA (rDNA)

DNA formed by combining sequences from two sources

Pharming

Using livestock to produce pharmaceuticals

Gene therapy

Genetic engineering to correct genetic errors in humans

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