Cloning and Biotechnology (OCR A-Level Biology A): Quizzes

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Immobilised Enzymes
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What economic limitation led to the development of enzyme immobilisation technology?

High cost of recovering soluble enzymes

What defines an immobilised enzyme?

Fixed to inert substance or in a bead

In the calcium alginate method, what happens when the enzyme-alginate mixture contacts calcium ions?

Viscous beads form immediately

Which immobilised enzyme converts glucose to fructose in high-fructose corn syrup production?

Glucose isomerase

What is a key advantage of using immobilised enzymes over soluble enzymes in industry?

No contamination of final product

Why is glucose isomerase expensive to extract from microorganisms?

It is an intracellular enzyme

Which test gives a negative result to confirm no enzyme leakage from immobilised enzyme beads?

Biuret solution test for protein

Why do immobilised enzymes work more slowly than soluble enzymes?

Substrates must diffuse through gel matrix

At 50°C, immobilised lactase maintains approximately what percentage of its maximum activity?

70%

Why are immobilised enzymes more resistant to denaturation at high temperatures?

Gel matrix provides structural support

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