Cloning (HSC SSCE Biology): Flashcards

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Cloning
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Cloning

Creating genetically identical copies of organism or gene

Gene cloning purpose

Create multiple identical copies of a specific gene

Restriction enzymes function

Cut DNA at specific sequences like molecular scissors

Recombinant DNA

DNA formed by joining a gene to a plasmid using ligase

PCR (Polymerase Chain Reaction)

Rapidly amplifies specific DNA sequences in vitro

Taq polymerase source

Thermus aquaticus, a bacterium from hot springs

PCR amplification after nn cycles

2n2^n copies produced

First cloned mammal from adult cell

Dolly the sheep (5 July 1996)

SCNT clone's genetic identity

Identical to nucleus donor, not egg donor or surrogate

Cloning effect on genetic diversity

Reduces diversity, making populations vulnerable to disease

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