The Electron Microscope (AQA A-Level Biology): Flashcards

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The Electron Microscope
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Why do electron microscopes have better resolution than light microscopes?

Shorter wavelength of electron beam

Resolution improvement of electron microscopes over light microscopes

2000 times better

Why is a vacuum needed in electron microscopes?

Electrons interact with air molecules

TEM stands for

Transmission electron microscope

SEM stands for

Scanning electron microscope

TEM resolving power

0.1 nm0.1 \text{ nm}

SEM resolving power

20 nm20 \text{ nm}

What are artefacts in electron microscopy?

Artificial features from preparation, not in natural specimen

Image type: TEM vs SEM

TEM: 2-D; SEM: 3-D surface

Why can't living specimens be observed in electron microscopes?

Vacuum requirement

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