The Electron Microscope (AQA A-Level Biology): Quizzes

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The Electron Microscope
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Why do electron beams provide better resolution than light in microscopy?

They have a much shorter wavelength

How much better is the resolution of modern electron microscopes compared to light microscopes?

2000 times better

Why must specimens be placed in a near-vacuum for electron microscopy?

Electrons interact with air molecules

What is used to focus the electron beam in an electron microscope?

Electromagnets

What is the resolving power of a transmission electron microscope (TEM)?

0.1 nm0.1 \text{ nm}

What is the resolving power of a scanning electron microscope (SEM)?

Around 20 nm20 \text{ nm}

How is contrast created in TEM images?

Differential electron absorption

What are artefacts in electron microscopy?

Artificial features from preparation

What type of images do TEM and SEM produce?

TEM: 2-D images; SEM: 3-D images

Why do SEM specimens not need to be extremely thin unlike TEM specimens?

Electrons don't penetrate in SEM

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