Chromatography (A-level only) (AQA A-Level Chemistry): Flashcards

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Chromatography
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What is chromatography?

Separation technique to separate & identify mixture components

Mobile phase in chromatography

The solvent that dissolves the mixture

Stationary phase in chromatography

The solid through which mobile phase passes

TLC (Thin-layer chromatography)

Plate coated with solid, solvent moves up

Column chromatography (CC)

Column packed with solid, solvent moves down

Gas chromatography (GC)

Column packed with solid; gas passed through under pressure at high temp

Retention time

Time for substance to pass through stationary phase

Effect of particle size on retention time

Larger particles move more slowly

Effect of solubility on retention time

More soluble in stationary = slower, in mobile = faster

Effect of adsorption on retention time

Greater attraction to stationary = slower movement

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