Studying Cells by Microscopy (OCR A-Level Biology A): Flashcards

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Studying Cells by Microscopy
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First to observe cells & when

Robert Hooke, 1660s

TEM max magnification & resolution

×500,000 & 0.2 nm

SEM max magnification & resolution

×100,000 & 10 nm

Light microscope max magnification & resolution

×1500 & 200 nm

Magnification formula

Image length ÷ real length

Resolution definition

Ability to distinguish two adjacent points as separate

TEM vs SEM: what each shows

TEM: internal ultrastructure; SEM: surface details/3D

Laser confocal microscope advantage

Eliminates blur from out-of-focus tissue

Total magnification (light microscope)

Eyepiece magnification × objective magnification

Why specimens need staining

Tissue is transparent; stains make structures visible

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