Immobilised Enzymes (OCR A-Level Biology A): Flashcards
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Immobilised enzyme definition
Immobilised enzyme definition
Enzyme fixed to inert substance/bead/membrane, not free in solution
Why enzyme immobilisation developed
Why enzyme immobilisation developed
High cost of recovering & reusing soluble enzymes after single use
Calcium alginate immobilisation method
Calcium alginate immobilisation method
Sodium alginate + enzyme dripped into CaCl2; forms gel bead matrix
Glucose isomerase industrial use
Glucose isomerase industrial use
Converts glucose to fructose in HFCS production
Lactase industrial use
Lactase industrial use
Breaks down lactose in milk for lactose-intolerant people
Immobilised enzyme advantage: reusability
Immobilised enzyme advantage: reusability
Enzymes can be reused multiple times before replacement
Immobilised enzyme advantage: product purity
Immobilised enzyme advantage: product purity
No contamination of final product with enzyme protein
Immobilised enzyme advantage: temperature
Immobilised enzyme advantage: temperature
Greater tolerance of temperature extremes than soluble enzymes
Why immobilised enzymes more thermally stable
Why immobilised enzymes more thermally stable
Gel matrix provides structural support, maintains protein structure
HFCS composition
HFCS composition
Approximately 50% fructose and 50% glucose