Core Practical: Investigating rate of enzymes (Edexcel GCSE Biology Combined Science): Flashcards

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Core Practical: Investigating rate of enzymes
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Product when amylase breaks down starch

Maltose

Indicator used to detect starch

Iodine

Iodine colour in presence of starch

Blue-black

Aim of the amylase practical

Determine optimum pH for amylase activity

Temperature kept constant during practical

35°C

Volume of amylase solution used

3cm³

Sampling interval for testing starch breakdown

Every 10 seconds

Rate calculation formula (measuring time only)

1000/time1000/time

Optimum pH for amylase activity

pH 7

Iodine colour when starch is absent

Browny-orange

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