DNA Technologies (HSC SSCE Biology): Flashcards

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DNA Technologies
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Human genome: base pairs & genes (approx.)

~3 billion base pairs, ~21,000 genes

DNA sequencing definition

Determining precise order of nucleotides (A, T, G, C) in DNA

DNA profiling definition

Determining organism's unique DNA profile as series of bands

Sanger method alternative name

Dideoxy DNA (ddDNA) sequencing

ddNTPs function

Chain-terminating nucleotides that stop DNA synthesis

STRs (Short Tandem Repeats)

Non-coding DNA sections with repeated sequences that vary

Developer of DNA profiling

Sir Alec Jeffreys

Chance of identical DNA profile (unrelated people)

Less than 1 in 1 billion

Gel electrophoresis: which fragments move faster?

Shorter fragments move faster; longer fragments slower

PCR (polymerase chain reaction) purpose

Amplifies/increases amount of DNA

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