Measuring Enzyme-Catalysed Reactions (AQA A-Level Biology): Flashcards
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Practise the cards
10 cards from this deck
Two requirements for enzyme function
Two requirements for enzyme function
Physical contact with substrate & correct active site shape
Time-course of enzyme reaction
Time-course of enzyme reaction
Monitoring how reaction progresses over time
Two approaches to measure enzyme reactions
Two approaches to measure enzyme reactions
Product formation & substrate disappearance measurement
Product formation measurement
Product formation measurement
Monitoring accumulation of reaction products over time
Substrate disappearance measurement
Substrate disappearance measurement
Tracking reduction in substrate concentration over time
Why enzyme reaction rate decreases over time
Why enzyme reaction rate decreases over time
Substrate depletion & product accumulation
What steep initial slope indicates
What steep initial slope indicates
High reaction rate at start
What graph plateau indicates
What graph plateau indicates
Reaction completion; substrate exhausted
Example: catalase enzyme reaction
Example: catalase enzyme reaction
Breaks down hydrogen peroxide, producing oxygen gas
Why is initial phase of enzyme reaction fastest?
Why is initial phase of enzyme reaction fastest?
High substrate concentration & all active sites available