Factors Affecting Enzyme Action (AQA A-Level Biology): Quizzes

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Factors Affecting Enzyme Action
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When investigating temperature effects on enzymes, which variables must be kept constant?

pH, enzyme concentration, substrate concentration

How is the relationship between an enzyme and its substrate best described?

Complementary

Why does increasing temperature initially increase enzyme reaction rate?

Raises kinetic energy, more collisions

At what temperature does denaturation typically occur for most enzymes?

Approximately 60°C

What happens to enzyme activity at the optimum temperature?

Reaction rate is at its maximum

How do pH changes affect enzyme active sites?

Alter charges on amino acid residues

What effect do small pH shifts typically have on enzymes in living organisms?

Reduce activity temporarily

What happens when enzyme concentration increases while substrate is in excess?

Reaction rate increases proportionally

What happens when enzyme concentration increases but substrate is the limiting factor?

No effect on reaction rate

What is VmaxV_{max} in relation to substrate concentration?

Maximum rate when all sites saturated

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