Factors Affecting Enzyme Action (AQA A-Level Biology): Flashcards
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Denaturation
Denaturation
Irreversible loss of enzyme shape and function
Optimum temperature
Optimum temperature
Temperature where enzyme works best
Optimum pH
Optimum pH
pH where enzyme functions most effectively
Vmax
Vmax
Maximum velocity when all active sites saturated
Enzyme-substrate relationship
Enzyme-substrate relationship
Complementary, like lock and key
Effect of temperature increase on molecules
Effect of temperature increase on molecules
Raises kinetic energy, more collisions
Formula for pH
Formula for pH
Enzyme concentration effect (excess substrate)
Enzyme concentration effect (excess substrate)
More active sites, higher rate
At substrate saturation
At substrate saturation
All active sites occupied, reaches Vmax
Reversibility of denaturation
Reversibility of denaturation
No, it is permanent and irreversible
