Factors Affecting Enzyme Action (AQA A-Level Biology): Flashcards

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Factors Affecting Enzyme Action
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Denaturation

Irreversible loss of enzyme shape and function

Optimum temperature

Temperature where enzyme works best

Optimum pH

pH where enzyme functions most effectively

Vmax

Maximum velocity when all active sites saturated

Enzyme-substrate relationship

Complementary, like lock and key

Effect of temperature increase on molecules

Raises kinetic energy, more collisions

Formula for pH

pH=log10[H+]pH = -\log_{10}[H^+]

Enzyme concentration effect (excess substrate)

More active sites, higher rate

At substrate saturation

All active sites occupied, reaches Vmax

Reversibility of denaturation

No, it is permanent and irreversible

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