Thin Layer Chromatography (AQA A-Level Chemistry): Flashcards

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Thin Layer Chromatography
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Stationary phase in TLC

Alumina or silica gel on a plate

Mobile phase in TLC

Organic solvent

How solvent moves up TLC plate

Capillary action

Molecules with strong mobile phase attraction

Travel more quickly up the plate

Molecules with strong stationary phase attraction

Move more slowly up the plate

Purpose of RfR_f values in TLC

Identifying substances

RfR_f value formula

Rf=distance moved by spotdistance moved by solventR_f = \frac{\text{distance moved by spot}}{\text{distance moved by solvent}}

Solvent front in TLC

Top point where solvent reaches

Making some TLC spots visible

UV light

Developing aromatic compounds in TLC

Iodine solution

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Optical Isomerism (A-level only)

Aldehydes & Ketones (A-level only)

Carboxylic Acids & Derivatives (A-level only)

Aromatic Chemistry (A-level only)

Amines (A Level only)

Polymers (A Level only)

Amino acids, Proteins & DNA (A Level only)

Organic Synthesis (A Level only)

Organic Mechanisms (A Level only)

Nuclear Magnetic Resonance Spectroscopy (A Level only)

Chromatography (A-level only)

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Organic Mechanisms (A Level only)

Nuclear Magnetic Resonance Spectroscopy (A Level only)

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