DNA Sequencing and Profiling (OCR A-Level Biology A): Flashcards
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DNA sequencing purpose
DNA sequencing purpose
Determines order of nucleotide bases (A, T, C, G)
Alternative name for chain-termination method
Alternative name for chain-termination method
Sanger sequencing
Function of dideoxynucleotides in sequencing
Function of dideoxynucleotides in sequencing
Lack 3' hydroxyl group; terminate DNA synthesis
NGS speed vs previous techniques
NGS speed vs previous techniques
100 to 1000 times faster
Pyrosequencing detection mechanism
Pyrosequencing detection mechanism
Luciferase emits light when nucleotide added
STR structure (short tandem repeats)
STR structure (short tandem repeats)
2-5 nucleotides repeated 10-30 times
European DNA 17 profile composition
European DNA 17 profile composition
16 STR loci plus sex markers
SNPs percentage of human genetic variation
SNPs percentage of human genetic variation
Around 90%
SNP frequency in human genome
SNP frequency in human genome
About 10 million; one every 100-300 base pairs
Personalised medicine definition
Personalised medicine definition
Treatment tailored to individual's genetic makeup