Techniques in DNA Analysis (OCR A-Level Biology A): Flashcards
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Practise the cards
10 cards from this deck
Exons
Exons
Coding sequences translated into amino acids
Introns
Introns
Non-coding sequences removed during mRNA processing
PCR denaturation temp
PCR denaturation temp
94-95°C
PCR extension temp
PCR extension temp
72°C
Taq polymerase key property
Taq polymerase key property
Thermostable - active at high PCR temps
DNA copies after n PCR cycles
DNA copies after n PCR cycles
2^n
DNA movement in gel electrophoresis
DNA movement in gel electrophoresis
Toward anode (DNA is negatively charged)
DNA fragment size vs distance in gel
DNA fragment size vs distance in gel
Shorter fragments travel further
SDS-PAGE effect on proteins
SDS-PAGE effect on proteins
Denatures into negative rods, separates by size
Multiplex PCR
Multiplex PCR
Amplifies multiple DNA sequences simultaneously