Techniques in DNA Analysis (OCR A-Level Biology A): Quizzes
📚Quizzes
Practise the questions
10 questions from this quiz
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Practise the questions
10 questions from this quiz
What are exons in structural genes?
What are exons in structural genes?
Coding sequences translated into amino acids
A gene has 525 bp of exons. How many amino acids does the polypeptide contain?
A gene has 525 bp of exons. How many amino acids does the polypeptide contain?
175 amino acids
What temperature is used during the denaturation stage of PCR?
What temperature is used during the denaturation stage of PCR?
94-95°C
What are the two critical functions of primers in PCR?
What are the two critical functions of primers in PCR?
Identify synthesis start & provide dsDNA region
Why is Taq polymerase used in PCR?
Why is Taq polymerase used in PCR?
It remains active at high temperatures
How many DNA copies are produced after 20 PCR cycles?
How many DNA copies are produced after 20 PCR cycles?
1,048,576 copies
How does fragment length affect DNA movement in gel electrophoresis?
How does fragment length affect DNA movement in gel electrophoresis?
Shorter fragments move faster and further
What effect does SDS treatment have on proteins in PAGE?
What effect does SDS treatment have on proteins in PAGE?
Denatures them into negatively charged rods
Which electrode do DNA fragments move toward during electrophoresis?
Which electrode do DNA fragments move toward during electrophoresis?
Anode (positive electrode)
How does shotgun sequencing assemble DNA fragments?
How does shotgun sequencing assemble DNA fragments?
Compares sequences to find overlapping regions