Core Practical: Drawing specimen from microscope (Edexcel GCSE Biology): Flashcards

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Core Practical: Drawing specimen from microscope
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Definition of resolution in microscopy

How well a microscope distinguishes 2 points close together

Tool used to measure cell size in microscope

Eyepiece graticule

What calibrates the eyepiece graticule?

Stage micrometer

Formula for cell size using graticule

Divisions×Calibration Value\text{Divisions} \times \text{Calibration Value} (in μm\mu m)

How to calculate total magnification

Eyepiece magnification ×\times objective lens magnification

Which objective lens to start with?

Lowest-powered objective lens

Drawing guidelines for microscope specimens

Sharp pencils, smooth lines, no shading, label features

Purpose of low-power magnification (e.g. 10x10x)

Broad view of tissue and general arrangement of cells

Purpose of high-power magnification (e.g. 40x40x)

Observe individual cells in detail

Why add stain to transparent specimens?

Makes them easier to see

What to include on your microscope drawing?

Magnification, scale bar, labels of features

Function of coarse adjustment knob

Move stage up/down to bring specimen into focus

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