Core Practical: Investigating rate of enzymes (Edexcel GCSE Biology): Flashcards

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Core Practical: Investigating rate of enzymes
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Rate equation when measuring change over time

rate=change/timerate = change/time

Enzyme used in starch breakdown practical

Amylase

Product when amylase breaks down starch

Maltose

Indicator used to detect starch

Iodine solution

Colour of iodine with starch present

Blue-black

Colour of iodine when starch absent

Browny-orange

Temperature maintained in water bath

35°C

Volume of amylase solution used

3 cm³

Volume of buffer solution added

1 cm³

Volume of starch solution added

3 cm³

Time interval for continuous sampling

Every 10 seconds

Rate formula for time-based experiments

1000/time1000/time

Optimum pH for amylase activity

pH 7

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