High-Performance Liquid Chromatography (VCE SSCE Chemistry): Flashcards

Separate and identify components in mixtures

Moving phase carrying sample (liquid or gas)

Non-moving fixed phase (solid or liquid on solid)

Different polarities cause different adsorption rates

Time for component to pass through column

$3-5$ $\mu$m

Non-polar (modified silica with hydrocarbon chains)

Polar solvent (e.g. water/methanol)

Polar molecules

UV light absorption