High-Performance Liquid Chromatography (VCE SSCE Chemistry): Quizzes
📚Quizzes
Practise the questions
10 questions from this quiz
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Practise the questions
10 questions from this quiz
What is the mobile phase in chromatography?
What is the mobile phase in chromatography?
The moving phase carrying the sample
Why do components with different polarities separate in chromatography?
Why do components with different polarities separate in chromatography?
They adsorb/desorb at different rates
What is retention time () in column chromatography?
What is retention time () in column chromatography?
Time for component to pass through column
What is the lowest concentration HPLC can detect?
What is the lowest concentration HPLC can detect?
Parts per trillion
What is the typical particle size in HPLC stationary phase?
What is the typical particle size in HPLC stationary phase?
3-5 micrometres
What type of stationary phase is used in reversed-phase HPLC?
What type of stationary phase is used in reversed-phase HPLC?
Non-polar modified silica
What type of mobile phase is used in reversed-phase HPLC?
What type of mobile phase is used in reversed-phase HPLC?
Polar solvent like water/methanol
Which molecules elute faster in reversed-phase HPLC?
Which molecules elute faster in reversed-phase HPLC?
Polar molecules
What detection method is commonly used in HPLC?
What detection method is commonly used in HPLC?
Ultraviolet light absorption
What happens when spiking confirms a compound's identity in HPLC?
What happens when spiking confirms a compound's identity in HPLC?
Peak grows larger, no new peaks appear