Evidence for Semi-conservative Replication (AQA A-Level Biology): Quizzes

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Evidence for Semi-conservative Replication
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In which year did Watson and Crick determine the structure of DNA?

1953

In the conservative model, what happens to the original DNA molecule during replication?

It remains completely intact

Which two nitrogen isotopes did Meselson and Stahl use in their experiment?

14N^{14}N and 15N^{15}N

What technique did Meselson and Stahl use to separate different DNA types?

Density gradient centrifugation

Where does heavier DNA settle during density gradient centrifugation?

Near the bottom of the tube

What density did all DNA show after one generation in 14N^{14}N medium (Generation 1)?

Intermediate density

What proportion of DNA was light density in Generation 2?

50%

Why did Generation 1 results rule out the conservative model?

DNA showed single intermediate band

In semi-conservative replication, what does each new DNA molecule contain?

One original and one new strand

Which base pairs with adenine in complementary base pairing?

Thymine

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