Column Chromatography (AQA A-Level Chemistry): Flashcards

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Column Chromatography
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Basis of separation in column chromatography

Differing levels of adsorption to adsorbent

Speed of lower adsorption components in column

Faster

Speed of higher adsorption components in column

Slower

Components eluted first in column chromatography

Those that travel faster (lower adsorption)

Rf formula

Rf=solute distancesolvent distanceRf = \frac{\text{solute distance}}{\text{solvent distance}}

Elution in chromatography

Removal of adsorbed molecules by solvent exchange

Eluent in chromatography

Mobile phase (solvent) flowing through column

Eluate in chromatography

Mixture exiting: mobile phase + separated components

Mobile phase in column chromatography

Liquid solvent (water or organic solvents)

Separation basis in gel column chromatography

Size of molecules

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