Core Practical: Investigating antibiotics & antiseptics (Edexcel GCSE Biology Combined Science): Quizzes

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Core Practical: Investigating antibiotics & antiseptics
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How are glass Petri dishes and agar gel sterilised for this practical?

In an autoclave

What is the purpose of the agar in the Petri dish?

Provides a nutrient-rich environment

Why is a sterile spreader used to spread the bacterial solution?

To create a uniform bacterial lawn

Why are the Petri dishes stored upside down?

Stops condensation contaminating culture

What is the maximum temperature for incubating plates in schools?

25°C

Why are plates not fully sealed with tape?

To allow oxygen in

What are the filter paper disks soaked in?

Antibiotics, antiseptics or extracts

What is the zone of inhibition?

Clear area where bacteria can't grow

What does a larger zone of inhibition indicate?

More effective antimicrobial agent

What is the purpose of the control disk?

To ensure accurate comparisons

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