Core Practical: Investigating antibiotics & antiseptics (Edexcel GCSE Biology Combined Science): Flashcards

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Core Practical: Investigating antibiotics & antiseptics
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What are agar plates used for in this practical?

Testing effectiveness of antibiotics/antiseptics/plant extracts

Equipment to sterilise Petri dishes & agar

Autoclave

Reason for sterilising equipment

Prevents unwanted bacteria contaminating the experiment

Max incubation temp in schools

25°C

Reason for 25°C max in schools

Prevents growth of harmful pathogens

Zone of inhibition

Clear area around disk where bacteria killed or growth prevented

Meaning of larger zone of inhibition

More effective antimicrobial agent

Reason for storing plates upside down

Stops condensation contaminating bacterial culture

Reason for partial (not full) sealing

Allows oxygen in, prevents anaerobic bacteria growth

Purpose of control disk

Ensures accurate comparisons

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