Genetic engineering (Edexcel GCSE Biology): Flashcards

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Genetic engineering
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What is genetic engineering?

Altering an organism's genetic material by introducing a gene from another organism to give it a desired characteristic

What do restriction enzymes do?

Cut DNA at specific sequences, creating sticky ends

What do ligase enzymes do?

Join DNA pieces together at their sticky ends

What is recombinant DNA?

Two pieces of DNA stuck together

What is a vector in genetic engineering?

Something used to transfer DNA into a cell

What are plasmids as vectors?

Small circular DNA molecules transferred between bacteria

How do viruses work as vectors?

Insert DNA into organisms they infect

What are sticky ends?

Unpaired bases left when DNA is cut by restriction enzymes

Purpose of antibiotic resistance marker

Identifies bacteria with successful genetic modifications

Main benefits of GM crops

Insect resistance, herbicide tolerance, higher yields

What is gene therapy?

Introducing normal genes into cells to correct genetic disorders by producing correct proteins

How is insulin produced via genetic engineering?

Microorganisms genetically modified to mass produce insulin

GM crops environmental risk

Might affect wild plants/insects, reducing biodiversity

Super weeds and pests risk from GM crops

GM crops could create resistance to herbicides and pesticides

Human antibodies from GM animals

Produced in sheep/cows for therapy (arthritis, cancer, MS)

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