CRISPR-Cas9 (VCE SSCE Biology): Flashcards
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CRISPR acronym meaning
CRISPR acronym meaning
Clustered Regularly Interspaced Short Palindromic Repeats
Bacteriophage definition
Bacteriophage definition
A virus that infects prokaryotic organisms
Type of enzyme Cas9 is
Type of enzyme Cas9 is
Endonuclease that creates blunt end cuts in DNA
PAM sequence function
PAM sequence function
Helps Cas9 locate target DNA & protects bacterial genome
3 stages of bacterial CRISPR defense
3 stages of bacterial CRISPR defense
Exposure, Expression, Extermination
Spacer in CRISPR
Spacer in CRISPR
Viral DNA sequence stored in bacterial CRISPR for defence
sgRNA in gene editing
sgRNA in gene editing
Single guide RNA synthesised in lab to direct Cas9 cuts
Blunt end cut
Blunt end cut
Straight DNA cut with no overhanging nucleotides
Gene knockout purpose
Gene knockout purpose
Preventing expression of a target gene
Off-target cleavage meaning
Off-target cleavage meaning
Unintended DNA edits at wrong genome locations