Recombination and Transformation (VCE SSCE Biology): Quizzes
📚Quizzes
Practise the questions
10 questions from this quiz
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Practise the questions
10 questions from this quiz
What are plasmids?
What are plasmids?
Small circular DNA loops in bacteria
Why must introns be removed from genes before inserting them into bacteria?
Why must introns be removed from genes before inserting them into bacteria?
Bacteria lack machinery to remove introns
Which four components are essential for making a recombinant plasmid?
Which four components are essential for making a recombinant plasmid?
Gene, plasmid, restriction enzyme, ligase
What is the function of a reporter gene in a plasmid vector?
What is the function of a reporter gene in a plasmid vector?
Identify successful gene insertion
What does the heat shock method use to increase bacterial membrane permeability?
What does the heat shock method use to increase bacterial membrane permeability?
Rapid temperature changes
What is the function of DNA ligase in creating recombinant plasmids?
What is the function of DNA ligase in creating recombinant plasmids?
Joins gene to plasmid via bonds
Why are bacteria cultured on antibiotic-rich agar plates after transformation?
Why are bacteria cultured on antibiotic-rich agar plates after transformation?
Only transformed bacteria survive
Why does insulin production require TWO different recombinant plasmids?
Why does insulin production require TWO different recombinant plasmids?
Insulin has two separate polypeptides
What is the function of the origin of replication (ORI) in a plasmid vector?
What is the function of the origin of replication (ORI) in a plasmid vector?
Signals where DNA replication begins
How are bacteria with recombinant plasmids identified using the gfp reporter gene?
How are bacteria with recombinant plasmids identified using the gfp reporter gene?
They do NOT glow under UV light