CRISPR-Cas9 (VCE SSCE Biology): Quizzes

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CRISPR-Cas9
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What does CRISPR stand for?

Clustered Regularly Interspaced Short Palindromic Repeats

What type of enzyme is Cas9?

An endonuclease that creates blunt end cuts

What are the three stages of the bacterial CRISPR-Cas9 defense system?

Exposure, expression, and extermination

What is the function of the PAM sequence in CRISPR-Cas9?

Helps Cas9 locate target DNA efficiently

What are spacers in the CRISPR sequence?

Short DNA sequences from invading viruses

What type of cut does Cas9 make in DNA?

A blunt end cut with no overhanging nucleotides

What is sgRNA in gene editing?

Synthetic single-stranded guide RNA made in labs

When must embryos be treated for successful whole-organism gene modification?

Before cells undergo differentiation

Which is an application of CRISPR-Cas9 in agriculture?

Introducing pest-resistance genes in crops

What is illegal in Australia regarding CRISPR-Cas9?

Implanting modified embryos into humans

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