The Polymerase Chain Reaction (VCE SSCE Biology): Quizzes
📚Quizzes
Practise the questions
10 questions from this quiz
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Practise the questions
10 questions from this quiz
What is the main purpose of PCR?
What is the main purpose of PCR?
To produce many identical copies of DNA
Which application uses PCR to analyze bodily fluids from crime scenes?
Which application uses PCR to analyze bodily fluids from crime scenes?
Forensic testing
Using the formula , how many DNA molecules are produced after 10 cycles?
Using the formula , how many DNA molecules are produced after 10 cycles?
1,024
Which material is NOT required for PCR?
Which material is NOT required for PCR?
RNA polymerase
At what temperature does denaturation occur during PCR?
At what temperature does denaturation occur during PCR?
90-95°C
What is the optimal temperature for Taq polymerase during elongation?
What is the optimal temperature for Taq polymerase during elongation?
72°C
Why is Taq polymerase used in PCR instead of human DNA polymerase?
Why is Taq polymerase used in PCR instead of human DNA polymerase?
Taq polymerase is heat-resistant
What happens during the annealing step of PCR?
What happens during the annealing step of PCR?
Primers bind to complementary sequences
Why does PCR require both forward and reverse primers?
Why does PCR require both forward and reverse primers?
DNA strands run in opposite directions
How many cycles are typically performed in a PCR run?
How many cycles are typically performed in a PCR run?
25-35 cycles