Gel Electrophoresis (VCE SSCE Biology): Quizzes

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Gel electrophoresis
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Why do smaller DNA fragments travel further than larger ones during gel electrophoresis?

Smaller fragments slip through pores easier

Why does DNA move toward the positive electrode during gel electrophoresis?

DNA has negative charge from phosphate

What material is the gel made from in gel electrophoresis?

Agarose

Which fluorescent dye is used to visualize DNA bands under UV light?

Ethidium bromide

What does a band positioned closer to the wells indicate?

Larger DNA fragments

What is the primary purpose of a standard ladder in gel electrophoresis?

Provide reference for fragment sizes

What does a thicker, more intense band indicate?

Greater concentration of DNA

How does higher voltage affect DNA migration in gel electrophoresis?

DNA migrates further

Why are STRs ideal for DNA profiling?

In non-coding regions, vary between people

How many bands appear for a heterozygous STR locus?

Two bands

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