Enzymes That Manipulate DNA (VCE SSCE Biology): Quizzes
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Practise the questions
10 questions from this quiz
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Practise the questions
10 questions from this quiz
What bond does an endonuclease break in a polynucleotide chain?
What bond does an endonuclease break in a polynucleotide chain?
Phosphodiester bond
What characteristic do recognition sites typically have?
What characteristic do recognition sites typically have?
They are palindromic sequences
Which enzyme creates sticky ends with overhanging nucleotides?
Which enzyme creates sticky ends with overhanging nucleotides?
EcoRI
A circular plasmid has 4 EcoRI recognition sites. How many fragments result after complete digestion?
A circular plasmid has 4 EcoRI recognition sites. How many fragments result after complete digestion?
4 fragments
Linear DNA with 3 recognition sites produces how many fragments after cutting?
Linear DNA with 3 recognition sites produces how many fragments after cutting?
4 fragments
How specific are ligases compared to restriction endonucleases?
How specific are ligases compared to restriction endonucleases?
Less specific - join any compatible ends
What does DNA polymerase require to begin synthesis?
What does DNA polymerase require to begin synthesis?
A primer to act as starting point
In what direction can polymerases synthesize DNA?
In what direction can polymerases synthesize DNA?
5' to 3' direction only
Where are restriction endonucleases naturally found?
Where are restriction endonucleases naturally found?
In bacteria as viral defense
Why is sticky end ligation more efficient than blunt end ligation?
Why is sticky end ligation more efficient than blunt end ligation?
Overhangs base pair, holding fragments