Enzymes That Manipulate DNA (VCE SSCE Biology): Flashcards
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3 main DNA manipulation enzymes
3 main DNA manipulation enzymes
Endonucleases (cut), ligases (paste), polymerases (copy)
Endonuclease function
Endonuclease function
Breaks phosphodiester bonds between nucleotides in DNA
Recognition site
Recognition site
Specific DNA sequence (4-6 nucleotides) where enzyme cuts
Palindromic recognition sequence property
Palindromic recognition sequence property
5' to 3' sequence identical on both DNA strands
Sticky ends vs blunt ends
Sticky ends vs blunt ends
Sticky: staggered cut with overhangs; Blunt: straight cut
Fragments from circular DNA with n cuts
Fragments from circular DNA with n cuts
n fragments
Fragments from linear DNA with n cuts
Fragments from linear DNA with n cuts
n+1 fragments
Ligase function
Ligase function
Joins DNA/RNA fragments by forming phosphodiester bonds
DNA polymerase synthesis direction
DNA polymerase synthesis direction
5' to 3' direction only
Primer in DNA synthesis
Primer in DNA synthesis
Short nucleic acid strand where polymerase starts synthesis