Gel Electrophoresis (VCE SSCE Biology): Flashcards
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Basis for DNA separation in gel electrophoresis
Basis for DNA separation in gel electrophoresis
Size
Material used to make the gel
Material used to make the gel
Agarose
Charge carried by DNA molecules
Charge carried by DNA molecules
Negative charge (due to phosphate backbone)
Size of fragments that travel furthest through gel
Size of fragments that travel furthest through gel
Smaller fragments
Fluorescent dye used to visualise DNA
Fluorescent dye used to visualise DNA
Ethidium bromide
Purpose of standard ladder in gel electrophoresis
Purpose of standard ladder in gel electrophoresis
Reference with DNA fragments of known lengths
What thicker bands indicate
What thicker bands indicate
Greater concentration/volume of DNA fragments
Units for measuring DNA fragment size
Units for measuring DNA fragment size
Base pairs (bp) or kilobases (kb)
Definition of DNA profiling
Definition of DNA profiling
Identification based on an individual's genetic information
What STRs are
What STRs are
Short tandem repeats - repeated nucleotide sections