Recombination and Transformation (VCE SSCE Biology): Flashcards
📚Flashcards
Practise the cards
10 cards from this deck
ShowHide
Practise the cards
10 cards from this deck
Plasmid
Plasmid
Small circular DNA loop separate from bacterial chromosome
Bacterial transformation
Bacterial transformation
Process where bacteria take up foreign DNA from environment
Why bacteria can't process genes with introns
Why bacteria can't process genes with introns
They lack cellular machinery to remove introns from mRNA
4 components to make recombinant plasmid
4 components to make recombinant plasmid
Gene of interest, plasmid vector, restriction enzyme, DNA ligase
Restriction endonuclease function
Restriction endonuclease function
Cuts DNA at specific recognition sites like molecular scissors
DNA ligase function
DNA ligase function
Joins DNA molecules by forming phosphodiester bonds
Heat shock method for transformation
Heat shock method for transformation
Rapid temp changes increase membrane permeability for DNA uptake
Electroporation method
Electroporation method
Electric shock increases membrane permeability for DNA uptake
Reporter gene purpose
Reporter gene purpose
Identifies successful genetic modification via observable trait
Why 2 plasmids needed for insulin production
Why 2 plasmids needed for insulin production
Insulin has 2 separate chains (alpha & beta) made separately