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10 cards from this deck
Lab technique to produce many identical copies of DNA
x=2nx = 2^nx=2n where x=DNA molecules, n=cycles
90-95°C (breaks H-bonds, separates DNA strands)
50-55°C (primers bind to complementary sequences)
72°C (Taq polymerase synthesises new DNA strand)
Heat-resistant DNA polymerase from Thermus aquaticus bacteria
Short single-stranded nucleic acids, starting points for enzyme
Paternity testing, forensic testing, genetic disease analysis
25-35 cycles
Heat-resistant, remains stable at high PCR temperatures
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