The Polymerase Chain Reaction (VCE SSCE Biology): Flashcards
📚Flashcards
Practise the cards
10 cards from this deck
ShowHide
Practise the cards
10 cards from this deck
PCR (polymerase chain reaction)
PCR (polymerase chain reaction)
Lab technique to produce many identical copies of DNA
PCR amplification formula
PCR amplification formula
where x=DNA molecules, n=cycles
PCR denaturation step temperature
PCR denaturation step temperature
90-95°C (breaks H-bonds, separates DNA strands)
PCR annealing step temperature
PCR annealing step temperature
50-55°C (primers bind to complementary sequences)
PCR elongation step temperature
PCR elongation step temperature
72°C (Taq polymerase synthesises new DNA strand)
Taq polymerase
Taq polymerase
Heat-resistant DNA polymerase from Thermus aquaticus bacteria
Primers in PCR
Primers in PCR
Short single-stranded nucleic acids, starting points for enzyme
Three applications of PCR
Three applications of PCR
Paternity testing, forensic testing, genetic disease analysis
Typical number of PCR cycles
Typical number of PCR cycles
25-35 cycles
Why Taq polymerase is used in PCR
Why Taq polymerase is used in PCR
Heat-resistant, remains stable at high PCR temperatures