Gene Expression (VCE SSCE Biology): Flashcards

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Gene expression
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Gene expression

Process creating functional products (proteins) from genes

3 stages of gene expression in eukaryotes

Transcription, RNA processing, Translation

Transcription

DNA copied into pre-mRNA by RNA polymerase

RNA polymerase function

Enzyme synthesising pre-mRNA from DNA template strand

Template strand vs coding strand

Template: transcribed; Coding: same sequence as mRNA (T→U)

RNA processing purpose

Adds 5' cap & 3' tail; removes introns; joins exons

Exons vs Introns

Exons: coding regions; Introns: non-coding regions

Translation

mRNA decoded into polypeptide chain at ribosomes

Codon

Three nucleotides in mRNA coding for one amino acid

Start codon

AUG - signals start of translation, codes for methionine

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