Aseptic technique (Edexcel GCSE Biology): Flashcards
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Aseptic techniques
Aseptic techniques
Methods to prevent contamination & maintain sterile conditions
What is an autoclave?
What is an autoclave?
Machine that sterilises using high-pressure steam
Temperature used in autoclave
Temperature used in autoclave
121°C
Items sterilised in autoclave
Items sterilised in autoclave
Petri dishes, culture vials, growth medium (agar/broth)
Purpose of inoculating loop
Purpose of inoculating loop
Transfer microorganisms from one place to another
How to sterilise inoculating loop
How to sterilise inoculating loop
Heat in Bunsen burner flame until red-hot
Why keep Petri dishes covered
Why keep Petri dishes covered
Avoid contamination from airborne microorganisms
How much to lift Petri dish lids when working
How much to lift Petri dish lids when working
Only slightly
Why are plates sealed but not airtight?
Why are plates sealed but not airtight?
Some microorganisms require oxygen to grow
Why aseptic techniques? (contamination)
Why aseptic techniques? (contamination)
Prevents contamination, ensures only desired organism grows
Why aseptic techniques? (safety)
Why aseptic techniques? (safety)
Reduces risk of harmful pathogens escaping to environment
Why aseptic techniques? (results)
Why aseptic techniques? (results)
Ensures results are valid and reliable