Aseptic technique (Edexcel GCSE Biology): Quizzes

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Aseptic technique
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What is the main purpose of aseptic techniques in microbiology?

To prevent contamination of cultures

At what temperature does an autoclave operate?

Around 121°C

Which items are sterilised in an autoclave?

Petri dishes, culture vials & medium

How is an inoculating loop sterilised before use?

Heated in a Bunsen burner flame

What colour should an inoculating loop glow when properly sterilised?

Red-hot

Why should petri dishes be kept covered as much as possible?

To avoid airborne contamination

How much should a petri dish lid be lifted when transferring microorganisms?

Only slightly

Why are petri dishes not sealed fully airtight?

Some microorganisms need oxygen

What does sealing petri dishes help to prevent?

Pathogens escaping into environment

Why does autoclave sterilisation ensure accurate experimental results?

Only studied microorganisms will grow

How do aseptic techniques improve laboratory safety?

They reduce harmful pathogen release

What type of results do aseptic techniques ensure?

Valid and reliable results

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