Core Practical: Investigating antibiotics & antiseptics (Edexcel GCSE Biology): Quizzes

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Core Practical: Investigating antibiotics & antiseptics
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How must glass Petri dishes and agar gel be sterilised before use?

In an autoclave

Why is sterilisation of equipment necessary in this experiment?

To prevent unwanted bacterial contamination

What is the purpose of the agar in the Petri dishes?

Provides nutrient-rich environment for growth

Why is the bacterial solution spread evenly across the agar surface?

To ensure a uniform lawn of bacteria

Why are plates only partially sealed with tape, not fully sealed?

Allows oxygen in to prevent anaerobic growth

Why are the Petri dishes stored upside down after preparation?

To stop condensation contaminating culture

What is the maximum incubation temperature used in schools?

25°C

At what temperature may medical settings incubate plates?

37°C for faster bacterial growth

What is the zone of inhibition?

Clear area where bacteria can't grow

What does a larger zone of inhibition indicate?

A more effective antimicrobial agent

What is the purpose of including a control disk in the experiment?

To ensure accurate comparisons

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