Core Practical: Investigating antibiotics & antiseptics (Edexcel GCSE Biology): Flashcards
📚Flashcards
Practise the cards
15 cards from this deck
ShowHide
Practise the cards
15 cards from this deck
What is tested using agar plates & bacteria
What is tested using agar plates & bacteria
Antibiotics, antiseptics, plant extracts
How to sterilise Petri dishes & agar
How to sterilise Petri dishes & agar
Autoclave
Why sterilise equipment
Why sterilise equipment
Prevents unwanted bacteria contaminating experiment
Purpose of agar gel
Purpose of agar gel
Provides nutrient-rich environment for bacteria to grow
Tool used to spread bacteria on agar
Tool used to spread bacteria on agar
Sterile spreader
Why spread bacteria on agar
Why spread bacteria on agar
Ensures uniform lawn of bacteria across plate
Why partially seal plates (not fully)
Why partially seal plates (not fully)
Prevents contamination but allows oxygen in
Why store plates upside down
Why store plates upside down
Stops condensation from contaminating bacterial culture
Max incubation temp in schools
Max incubation temp in schools
25°C
Why plates incubated at 25°C in schools
Why plates incubated at 25°C in schools
Prevents growth of harmful pathogens
Incubation temp in medical settings
Incubation temp in medical settings
37°C (body temperature)
What filter paper disks are soaked in
What filter paper disks are soaked in
Antibiotic, antiseptic, or plant extract solutions
Zone of inhibition definition
Zone of inhibition definition
Clear area around disk where bacteria killed/growth prevented
What larger zone of inhibition indicates
What larger zone of inhibition indicates
More effective antimicrobial agent
Purpose of control disk
Purpose of control disk
Ensure accurate comparisons