4a, 4b, 4c, 4d & 4e – To Investigate Factors that Affect the Rate of an Enzyme-Catalysed Reaction (LC 2027) (Leaving Cert Biology): Flashcards
📚Flashcards
Practise the cards
15 cards from this deck
ShowHide
Practise the cards
15 cards from this deck
Role of enzymes in living organisms
Role of enzymes in living organisms
Biological catalysts that speed up chemical reactions
Substrate that catalase breaks down
Substrate that catalase breaks down
Hydrogen peroxide (H₂O₂)
Products when catalase acts on hydrogen peroxide
Products when catalase acts on hydrogen peroxide
Water and oxygen
What foam production indicates in catalase reaction
What foam production indicates in catalase reaction
Enzyme activity rate (more foam = faster activity)
Purpose of pH buffer in enzyme investigations
Purpose of pH buffer in enzyme investigations
Maintains constant pH throughout experiment
Optimal temperature for catalase activity
Optimal temperature for catalase activity
Around 37°C (maximum enzyme activity)
Effect of boiling on enzyme activity
Effect of boiling on enzyme activity
Destroys activity (causes denaturation)
Effect of low temperature on enzyme reaction rate
Effect of low temperature on enzyme reaction rate
Slow reaction rate due to reduced molecular movement
What enzyme specificity means
What enzyme specificity means
Each enzyme reacts with only one particular substrate
Part of enzyme that fits with specific substrate
Part of enzyme that fits with specific substrate
Active site
Four main factors affecting enzyme activity
Four main factors affecting enzyme activity
pH, temperature, substrate conc., enzyme conc.
What denaturation does to enzymes
What denaturation does to enzymes
Changes enzyme shape permanently, destroys activity
Safety concern with hydrogen peroxide
Safety concern with hydrogen peroxide
Irritant to skin and eyes; corrosive
Effect of increasing substrate concentration
Effect of increasing substrate concentration
Increases reaction rate until enzyme saturation
Effect of increasing enzyme concentration
Effect of increasing enzyme concentration
Increases reaction rate (more enzyme molecules available)