Investigate the effect of heat denaturation on the rate of enzyme activity (LC 2026) (Leaving Cert Biology): Quizzes
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Practise the questions
12 questions from this quiz
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Practise the questions
12 questions from this quiz
What substrate is used in the enzyme denaturation experiment?
What substrate is used in the enzyme denaturation experiment?
Hydrogen peroxide
Which enzyme is being investigated in this experiment?
Which enzyme is being investigated in this experiment?
Catalase
What is the purpose of the pH buffer in this experiment?
What is the purpose of the pH buffer in this experiment?
Keeps pH constant
What are the two purposes of washing-up liquid in this experiment?
What are the two purposes of washing-up liquid in this experiment?
Measure activity & break cell walls
What volume of celery is used in the experiment?
What volume of celery is used in the experiment?
10 cm³
What volume of hydrogen peroxide is added in the method?
What volume of hydrogen peroxide is added in the method?
5 cm³
How long are the cylinders kept in the water bath before mixing?
How long are the cylinders kept in the water bath before mixing?
2 minutes
What does the volume of foam indicate in this experiment?
What does the volume of foam indicate in this experiment?
Amount of oxygen produced
What volume of foam was produced at 30°C?
What volume of foam was produced at 30°C?
40 cm³
What volume of foam was produced at 100°C?
What volume of foam was produced at 100°C?
0 cm³
Why was a large volume of foam produced at 30°C?
Why was a large volume of foam produced at 30°C?
30°C is the optimum temperature
What does it mean when an enzyme becomes denatured?
What does it mean when an enzyme becomes denatured?
Lost its 3D, folded, globular shape