Isolate DNA from a plant tissue (LC 2026) (Leaving Cert Biology): Quizzes
📚Quizzes
Practise the questions
12 questions from this quiz
ShowHide
Practise the questions
12 questions from this quiz
What is the purpose of chopping the kiwi into small pieces?
What is the purpose of chopping the kiwi into small pieces?
To increase surface area
What is the role of washing-up liquid in the DNA extraction?
What is the role of washing-up liquid in the DNA extraction?
It dissolves the cell membranes
Why is salt added to the solution with the kiwi?
Why is salt added to the solution with the kiwi?
To help DNA clump together
At what temperature is the beaker placed in the water bath?
At what temperature is the beaker placed in the water bath?
60°C
How long should the beaker remain in the water bath at 60°C?
How long should the beaker remain in the water bath at 60°C?
15 minutes
What is the purpose of heating the mixture at 60°C?
What is the purpose of heating the mixture at 60°C?
To denature enzymes that break down DNA
How long should the ice-water bath cooling step last?
How long should the ice-water bath cooling step last?
5 minutes
For how long should the mixture be blended?
For how long should the mixture be blended?
No more than 3 seconds
How many drops of protease should be added to the boiling tube?
How many drops of protease should be added to the boiling tube?
2-3 drops
What property does DNA have in relation to cold ethanol?
What property does DNA have in relation to cold ethanol?
DNA is insoluble in cold ethanol
Where does DNA appear after adding ice-cold alcohol?
Where does DNA appear after adding ice-cold alcohol?
At the ethanol-filtrate boundary
What is the purpose of adding ice-cold alcohol?
What is the purpose of adding ice-cold alcohol?
To separate DNA from the filtrate