Demonstration of the digestion of starch during germination by a seed (LC 2026) (Leaving Cert Biology): Flashcards
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13 cards from this deck
Define germination
Define germination
Regrowth of embryo after dormancy in suitable conditions
Enzyme used to digest starch in seed germination
Enzyme used to digest starch in seed germination
Amylase
Purpose of amylase in germinating seeds
Purpose of amylase in germinating seeds
Digests starch for respiration
Why soak bean seeds overnight before experiment?
Why soak bean seeds overnight before experiment?
Softens testa coat & activates enzymes to start germination
Purpose of boiling one seed in the experiment
Purpose of boiling one seed in the experiment
Denatures enzymes, stops germination, acts as control
Incubation temperature for starch digestion experiment
Incubation temperature for starch digestion experiment
27°C
Incubation time for seed germination experiment
Incubation time for seed germination experiment
48 hours
Why incubate at 27°C?
Why incubate at 27°C?
Optimal temperature for plant enzymes
Chemical test for starch
Chemical test for starch
Iodine solution
Result when iodine added to germinated seed area
Result when iodine added to germinated seed area
Stays brown (no starch present)
Result when iodine added to boiled seed (control)
Result when iodine added to boiled seed (control)
Turns blue/black (starch present)
What does clear ring around germinated seed indicate?
What does clear ring around germinated seed indicate?
Area where starch has been digested
When does clear ring appear around germinating seed?
When does clear ring appear around germinating seed?
After 24 hours