Investigate the effect of heat denaturation on the rate of enzyme activity (LC 2026) (Leaving Cert Biology): Flashcards
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Practise the cards
14 cards from this deck
Substrate in enzyme experiment
Substrate in enzyme experiment
Hydrogen peroxide
Enzyme found in celery
Enzyme found in celery
Catalase
Role of pH buffer
Role of pH buffer
Keeps pH constant
Washing up liquid functions
Washing up liquid functions
Produces visible foam & breaks down cell wall
Volume of celery used
Volume of celery used
10 cm³
Volume of hydrogen peroxide used
Volume of hydrogen peroxide used
5 cm³
Volume of pH buffer used
Volume of pH buffer used
10 cm³
Water bath equilibration time
Water bath equilibration time
2 minutes
Foam volume produced at 30°C
Foam volume produced at 30°C
40 cm³
Foam volume produced at 100°C
Foam volume produced at 100°C
0 cm³
Optimum temperature for catalase
Optimum temperature for catalase
30°C
Effect of 100°C on enzyme
Effect of 100°C on enzyme
Denaturation
What enzyme denaturation means
What enzyme denaturation means
Loss of 3D, folded, globular shape
What foam volume measures
What foam volume measures
Enzyme activity (oxygen produced)