Investigation of the effect of pH on enzyme activity (LC 2026) (Leaving Cert Biology): Flashcards
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14 cards from this deck
Substrate used in pH enzyme expt
Substrate used in pH enzyme expt
Hydrogen Peroxide
Source of catalase enzyme
Source of catalase enzyme
Celery
Enzyme found in celery
Enzyme found in celery
Catalase
pH values tested in expt
pH values tested in expt
4, 7, 10, 13
Temperature kept constant
Temperature kept constant
30°C
Volume of celery used
Volume of celery used
10cm³
Volume of pH buffer used
Volume of pH buffer used
10cm³
Volume of hydrogen peroxide added
Volume of hydrogen peroxide added
5cm³
Time in water bath before mixing
Time in water bath before mixing
2 minutes
What is measured in expt
What is measured in expt
Volume of foam produced
What foam volume indicates
What foam volume indicates
Enzyme activity (amount of oxygen produced)
Foam volume at pH 10
Foam volume at pH 10
13cm³
Foam volume at pH 4, 7 and 13
Foam volume at pH 4, 7 and 13
0cm³
Optimal pH for catalase (from results)
Optimal pH for catalase (from results)
pH 10