Investigation of the effect of temperature on enzyme activity (LC 2026) (Leaving Cert Biology): Flashcards

30°C

Temperature at which enzymes work best

Can alter shape, leading to denaturation

Active site destroyed, can't act on substrate

Hydrogen peroxide

Celery

Keeps pH constant

$5 \text{ cm}^3$

2 minutes

Enzyme activity/amount of oxygen produced

$0 \text{ cm}^3$

$5 \text{ cm}^3$

$15 \text{ cm}^3$

$5 \text{ cm}^3$